RayBiotech/人类Fra1转录因子活性测定/1平板试剂盒/TFEH-Fra1-1
商品编号：
TFEH-FRA1-1
品牌：
RayBiotech
市场价：
¥0.00
美元价：
0.00
产品分类：
活体成像系统
公司分类：
IVIS_Imaging_System
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Antigen Information
Accession Number:
- P15407
Gene ID:
- 8061
Gene Symbols:
- FOSL1
- FRA1
Protein Name & Synonyms:
Fos-related antigen 1 (FRA-1)
Target Species:
- Human
Assay Format
Specificity:
The olionucleotide/antibody pair provided in this kit recognizes human Fra1 in whole lysates and nuclear extracts.
Number of Targets Detected:
1
Species Detected:
- Human
Compatible Sample Types:
- Cell Lysates
- Nuclear Extracts
Design Principle:
- Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
- Semi-Quantitative
Solid Support:
96-well Microplate
Product Specifications
Size:
1, 2, or 5 x 96-Well Strip Microplate Kit
Introduction
Activator protein-1 (AP-1) is a sequence-specific transcriptional activator composed of members of the Jun (c-Jun, JunB, and JunD) and Fos (c-Fos, FosB, Fra1, and Fra2) families in formats of homo- and heterodimers. These proteins belong to the bZIP group of DNA binding proteins with the ability to a common consensus sequence-defined AP-1-binding site. Jun and Fos proteins can also dimerize other basic leucine zipper proteins such as ATF, CCAAT enhancer-binding protein, Maf, and NF-E2. Jun-Jun and Jun-Fos dimers bind preferentially to TPA responsive element (TRE), whose consensus is TGAGTCA, whereas Jun-ATF dimers prefer to bind to the c-AMP-responsive element (CRE) whose consensus is TGAGCTCA. Inside cells, AP-1 activity is induced by an incredible diversity of signals, including growth factors, cellular stress, ionizing and ultraviolet irradiation, DNA damage, oxidative stress, neuronal depolarization antigen binding by T or B lymphocytes, and cytokines. The mechanisms involved in induction of AP-1 activity are either through changing the expression of AP-1 components or post-translation modification or both to regulate their trans-activity positively or negatively. For examples, stimulation by growth factors or by activating mutations in cytoplasmic effectors such as ras and raf, results in AP-1 activation by triggering the ERK signaling pathway. On the other hand, AP-1 responses to proinflammatory cytokines and UV radiation mostly dependent on two other MAPK cascades, JNK and p38 of MAP kinases. As results, the AP-1 regulates different target genes executing different biological functions such as cell proliferation, differentiation, apoptosis, or cell death.
Product Features
- Specific transcription factor-DNA binding assay
- Perfect alternative to EMSA
- Easy to perform in an ELISA format
- Non-radioactive assay
- High throughput (96-well plate format)
- Assay can be completed within 5 hours
Application Notes
Kit Components
- 96-well Strip Microplate pre-coated with DNA probes
- DNA Binding Buffer
- Positive Control Sample
- Specific Competitor DNA probe
- Non-specific Competitor DNA probe
- Assay Reagent
- DTT
- Wash Buffer
- Primary Antibody
- HRP-conjugated Secondary Antibody
- Antibody Diluent Buffer
- TMB One-Step Substrate Reagent
- Stop Solution
Other Materials Required
- Distilled or deionized water
- 100 ml and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Absorbent paper
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- Microplate reader capable of measuring absorbance at 450 nm
Protocol Outline
- Prepare all reagents and samples as instructed in the manual.
- Add 100 µl of sample or positive control to each well.
- Incubate 2 h at RT or O/N at 4 °C.
- Add 100 µl of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared HRP-secondary antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Typical Data
Figure 1Transcription factor assay of Fra1 from nuclear extracts of K562 cells or K562 cells treated with PMA (50 ng/ml) for 3 hr with the RayBio® Activity Assay Kit .
Figure 2Transcription factor assay of Fra1 from nuclear extracts of K562 cells or K562 cells treated with PMA (50 ng/ml) for 3 hr with the specific competitor or non-specific competitor. The result shows specific binding of Fra1 to the conserve binding site detected by using the RayBio® Fra1 TF Activity Assay Kit.
Storage/Stability
Upon receipt, the positive control should be removed and stored at -20° or -80°C. The remainder of the kit can be stored for up to 6 months at 2-8°C from the date of shipment. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: The kit can be used within one year if the whole kit is stored at -20°C upon receipt. Avoid repeated freeze-thaw cycles.
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Manual
SDS
品牌介绍
raybiotech致力于开发、研制、生产及销售以抗体芯片、ELISA/EIA、抗原抗体为主的酶免产品。率先开发了高品质的抗体和蛋白质芯片技术平台，蛋白质芯片能够同时有效地分析生物样品中参与炎症、血管生长、细胞生长、细胞凋亡及信号转导等数百种蛋白的表达水平。近400页的产品目录，主要涉及蛋白质芯片、抗体芯片、抗体、免疫试剂、诊断试剂等近万种，拥有先进的技术优势。许多发表在Nature、Nature Medicine、 Cell、 PNAS、Lancet等世界顶级期刊上的文章中均使用了瑞博奥的产品，公司的芯片技术和产品质量都得到了广泛的肯定。
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