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RayBiotech/定制人和小鼠磷酸化-4E-BP1（Thr36）和基于总4E-BP1细胞的ELISA/1平板试剂盒/CBEL-4EBP1-T36-1
RayBiotech/定制人和小鼠磷酸化-4E-BP1（Thr36）和基于总4E-BP1细胞的ELISA/1平板试剂盒/CBEL-4EBP1-T36-1
商品编号：
CBEL-4EBP1-T36-1
品牌：
RayBiotech
市场价：
¥0.00
美元价：
0.00
产品分类：
酚
公司分类：
hydroxybenzene
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Antigen Information
Accession Number:
- Q13541
- Q60876
Gene ID:
- 1978
Gene Symbols:
- EIF4EBP1
Protein Name & Synonyms:
Eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1) (eIF4E-binding protein 1) (Phosphorylated heat- and acid-stable protein regulated by insulin 1) (PHAS-I)
Target Species:
- Human
- Mouse
Assay Format
Pathway:
- Akt Signaling
- AMPK Signaling
- HER/ErbB Signaling
- IGF Signaling
- mTOR Signaling
- PI3K-AKT Signaling
Specificity:
This ELISA kit recognizes Human
Number of Targets Detected:
2
Species Detected:
- Human
- Mouse
Compatible Sample Types:
- Adherent Cell Culture
Design Principle:
- Cell-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
- Semi-Quantitative
Solid Support:
96-well Microplate
Product Specifications
Size:
1, 2, or 5 x 96-Well Strip Microplate Kit
Product Features
- Rapidly measure phosphorylated protein in adherent cell lines
- Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
- No sample lysis is needed
- Compatible with a standard ELISA plate reader
Application Notes
Kit Components
- Uncoated 96-well Strip Microplate
- Wash Buffers
- Fixing Solution
- Quenching Buffer
- Blocking Buffer
- Anti-phospho antibody
- Anti-pan antibody
- HRP-Conjugated Secondary Antibody
- TMB One-Step Substrate
- Stop Solution
Other Materials Required
- Distilled or deionized water
- 100 ml and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
Protocol Outline
- Seed 10,000-30,000 cells into each well and incubate overnight.
- Apply various treatment, inhibitors or activators according to manufacturer"s instructions.
- Add 100 µl of Fixing Solution into each well and incubate for 20 min at RT with shaking.
- Add 200 µl of prepared 1X Quenching Buffer and incubate 20 min at RT.
- Add 200 µl of Blocking Solution and incubate for 1 h at 37°C.
- Add 50 µl of 1X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT.
- Add 50 µl of prepared 1X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
Upon receipt, the kit should be stored at â20°C. Please use within 6 months from the date of shipment.
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Manual
SDS
品牌介绍
raybiotech致力于开发、研制、生产及销售以抗体芯片、ELISA/EIA、抗原抗体为主的酶免产品。率先开发了高品质的抗体和蛋白质芯片技术平台，蛋白质芯片能够同时有效地分析生物样品中参与炎症、血管生长、细胞生长、细胞凋亡及信号转导等数百种蛋白的表达水平。近400页的产品目录，主要涉及蛋白质芯片、抗体芯片、抗体、免疫试剂、诊断试剂等近万种，拥有先进的技术优势。许多发表在Nature、Nature Medicine、 Cell、 PNAS、Lancet等世界顶级期刊上的文章中均使用了瑞博奥的产品，公司的芯片技术和产品质量都得到了广泛的肯定。
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