RayBiotech/人ANP EIA/1平板试剂盒/EIA-ANP-1
商品编号：
EIA-ANP-1
品牌：
RayBiotech
市场价：
¥0.00
美元价：
0.00
产品分类：
RNA修饰酶
公司分类：
RNA_enzymes
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Antigen Information
Accession Number:
- P01160
Gene ID:
- 4878
Gene Symbols:
- ANP
- NPPA
- PND
Protein Name & Synonyms:
Natriuretic peptides A (CDD-ANF) (Prepronatriodilatin) [Cleaved into: Cardiodilatin-related peptide (CDP) Atrial natriuretic factor (ANF) (Atrial natriuretic peptide) (ANP)]
Assay Format
Detection Range:
0.1-1,000 pg/ml
Recommended Dilution (serum & plasma):
Human: 4X / Mouse: 4X / Rat: 4X
Sensitivity:
1.02 pg/ml
Specificity:
This kit targets the common sequence of human, mouse and rat, and thus may be used to detect ANP expression in all these species with high specificity and sensitivity. Cross Reactivity: This EIA kit shows no cross-reactivity with any of the cytokines tested: Ghrelin, Nesfatin, Angiotensin II, NPY and APC.
Number of Targets Detected:
1
Species Detected:
- Human
- Mouse
- Rat
Compatible Sample Types:
- Cell Culture Supernatants
- Serum
Design Principle:
- Competition-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
- Quantitative
Solid Support:
96-well Microplate
Product Specifications
Size:
1, 2, or 5 x 96-Well Strip Microplate Kit
Introduction
Atrial natriuretic peptide (ANP) is a 28-amino acid peptide hormone secreted by cardiac myocytes of the atrium. ANP plays an important role in the homeostatic regulation of body water, sodium, potassium and fat, by acting to reduce the water, sodium and adipose loads on the circulatory system, thus reducing blood pressure.ANP peptide contains a 17-amino acid ring which is formed by a disulfide bond between two cysteine residues at positions 7 and 23. ANP is closely related to BNP (brain natriuretic peptide) and CNP (C-type natriuretic peptide), which all share the same amino acid ring. The mechanism of ANP-induced vasodilatation is through binding to a specific set of ANP receptors. Receptor-agonist binding causes a reduction in blood volume and therefore a reduction in cardiac output and systemic blood pressure. Lipolysis is increased and renal sodium reabsorption is decreased. The overall effect of ANP on the body is to counter increases in blood pressure and volume caused by the renin-angiotensin system.In addition to its vasodilatation effect, ANP also serves as a adipokine. Studies have shown that ANP Increases the release of free fatty acids from adipose tissue, activates adipocyte plasma membrane NPR-A receptors, and increases intracellular cGMP levels that induce the phosphorylation of a hormone-sensitive lipase and perilipin A.
Product Features
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- User Manual
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Orbital shaker
- Aluminum foil
- Saran Wrap
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- SigmaPlot software (or other software that can perform four-parameter logistic regression models)
Protocol Outline
- Prepare all reagents, samples and standards as instructed.
- Add 100 µl detection antibody to each well.
- Incubate 1.5 h at RT or O/N at 4°C.
- Add 100 µl standard or sample to each well.
- Incubate 2.5 h at RT.
- Add 100 µl prepared streptavidin solution.
- Incubate 45 min at RT.
- Add 100 µl TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl Stop Solution to each well.
- Read plate at 450 nm immediately.
Storage/Stability
Standard, Biotinylated Atrial natriuretic peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
品牌介绍
raybiotech致力于开发、研制、生产及销售以抗体芯片、ELISA/EIA、抗原抗体为主的酶免产品。率先开发了高品质的抗体和蛋白质芯片技术平台，蛋白质芯片能够同时有效地分析生物样品中参与炎症、血管生长、细胞生长、细胞凋亡及信号转导等数百种蛋白的表达水平。近400页的产品目录，主要涉及蛋白质芯片、抗体芯片、抗体、免疫试剂、诊断试剂等近万种，拥有先进的技术优势。许多发表在Nature、Nature Medicine、 Cell、 PNAS、Lancet等世界顶级期刊上的文章中均使用了瑞博奥的产品，公司的芯片技术和产品质量都得到了广泛的肯定。
联络我们
