RayBiotech/人17-β-HSD1/HSD17B1 ELISA/1平板试剂盒/ELH-17bHSD1-1
商品编号：
ELH-17bHSD1-1
品牌：
RayBiotech
市场价：
¥0.00
美元价：
0.00
产品分类：
其它成像系统
公司分类：
Other_imaging
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Antigen Information
Accession Number:
- P14061
Gene ID:
- 3292
Protein Name & Synonyms:
Estradiol 17-beta-dehydrogenase 1 (EC 1.1.1.62) (17-beta-hydroxysteroid dehydrogenase type 1) (17-beta-HSD 1) (20 alpha-hydroxysteroid dehydrogenase) (20-alpha-HSD) (E2DH) (Placental 17-beta-hydroxysteroid dehydrogenase) (Short chain dehydrogenase/reductase family 28C member 1)
Assay Format
Detection Range:
0.9 ng/ml - 200 ng/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
0.9 ng/ml
Specificity:
This ELISA kit shows no cross-reactivity with the following cytokines tested: human ADAMTSL-1/Punctin-1, AMSH/STAMBP, Annexin V, BATF3, Bora, Cadherin-17, Caveolin-2, CD2, CD200 R1, CHST3, COMT, Cystatin SA, Dopamine beta-Hydroxylase, Desmin, Exostosin-like 3, Ficolin-1, FosB/GOS3, FRS2, GATA-5, GFAP, GLI-3, HepaCAM, ARNT/HIF-1 beta, IDO, Kallikrein 1, Kell, MDL-1/CLEC5A, NPDC-1, Numb, Olig2, p63/TP73L, Pax3, Semaphorin 4D, Sphingosine Kinase 1/SPHK1, TAZ/WWTR1, TC-PTP, Transglutaminase 3/TGM3, TPST2, TREML1/TLT-1.
Number of Targets Detected:
1
Species Detected:
- Human
Compatible Sample Types:
- Cell Culture Supernatants
- Plasma
- Serum
Design Principle:
- Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
- Quantitative
Solid Support:
96-well Microplate
Product Specifications
Size:
1, 2, or 5 x 96-Well Strip Microplate Kit
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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Manual
SDS
品牌介绍
raybiotech致力于开发、研制、生产及销售以抗体芯片、ELISA/EIA、抗原抗体为主的酶免产品。率先开发了高品质的抗体和蛋白质芯片技术平台，蛋白质芯片能够同时有效地分析生物样品中参与炎症、血管生长、细胞生长、细胞凋亡及信号转导等数百种蛋白的表达水平。近400页的产品目录，主要涉及蛋白质芯片、抗体芯片、抗体、免疫试剂、诊断试剂等近万种，拥有先进的技术优势。许多发表在Nature、Nature Medicine、 Cell、 PNAS、Lancet等世界顶级期刊上的文章中均使用了瑞博奥的产品，公司的芯片技术和产品质量都得到了广泛的肯定。
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