RayBiotech/快速小鼠免疫球蛋白同种型阵列1/16样本试剂盒/AAM-ISO-G1-1
商品编号：
AAM-ISO-G1-1
品牌：
RayBiotech
市场价：
¥0.00
美元价：
0.00
产品分类：
酯
公司分类：
Sphingomyelin
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Antigen Information
Gene Symbols:
- IGA
- IGE
- IGG2A
- IGHG1
- IGM
Assay Format
Number of Targets Detected:
10
Species Detected:
- Mouse
Compatible Sample Types:
- Cell Culture Supernatants
- Cell Lysates
- Plasma
- Serum
- Tissue Lysates
Design Principle:
- Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
- Semi-Quantitative
Solid Support:
Glass Slide
Product Specifications
Size:
16, 32, or 64 Sample Kit
Product Feature
- One step mouse monoclonal antibody isotyping
- Use only 1-2 µl sample
- The whole experiment can be done within 1 hour
- Sandwich based technology for high specificity and sensitivity
- Low system CV with high reproducibility
- High throughput sample processing
- Qualitative visual inspection or semi-quantitative result
- Processed slides can be stored for years without signal decay
Target Name
IgA, IgD, IgE, IgM, IgG1, IgG2a, IgG2b, and IgG3 Heavy Chains; Kappa and Lambda Light Chains
Target Species
Mouse
Application Notes
Suggested Application
Isotyping; Monoclonal Antibody Development; Hybridoma Screening and Selection; Biomarker Screening; Confirming Biological Processes
Kit Components
- Mouse Ig Rapid Isotyping Glass Slide(s)
- Sample Diluent
- 20X Wash Buffer
- Labeled Detection Antibody
- Slide Washer/Dryer
- 96-well Plate
- Manual
Other Materials Required
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline
- Dry the glass slide
- Mix samples with detection antibodies (in 96-well plate)
- Incubate with Sample/antibody mix (30 min at 37°C, followed by washes)
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
Upon receipt, all components of the Quantibody Array kit should be stored at -20 °C. At -20 °C the kit will retain complete activity for up to 6 months. The entire kit should be used within 6 months of purchase.
- Shao W., Zhang C., Liu E., et al. Identification of Liver Epithelial Cell-derived Ig Expression in ? chain-deficient mice. Sci Rep. 2016; 6: 23669. Published online 2016 Mar 29. doi: 10.1038/srep23669Species: MouseSample type: Tissue Lysate
- Groscurth S., Muller B., Schwan B., et al. Artificial forisomes are ideal models of forisome assembly and activity that allow the development of technical devices. Biomacromolecules. 2012 Oct 8;13(10):3076-86. doi: 10.1021/bm3008499Species: MouseSample type: Conditioned Media
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Manual
SDS
Analysis Tool
品牌介绍
raybiotech致力于开发、研制、生产及销售以抗体芯片、ELISA/EIA、抗原抗体为主的酶免产品。率先开发了高品质的抗体和蛋白质芯片技术平台，蛋白质芯片能够同时有效地分析生物样品中参与炎症、血管生长、细胞生长、细胞凋亡及信号转导等数百种蛋白的表达水平。近400页的产品目录，主要涉及蛋白质芯片、抗体芯片、抗体、免疫试剂、诊断试剂等近万种，拥有先进的技术优势。许多发表在Nature、Nature Medicine、 Cell、 PNAS、Lancet等世界顶级期刊上的文章中均使用了瑞博奥的产品，公司的芯片技术和产品质量都得到了广泛的肯定。
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