RayBiotech/Glycan Array 100/4样本试剂盒/GA-Glycan-100-1
商品编号：
GA-Glycan-100-1
品牌：
RayBiotech
市场价：
¥0.00
美元价：
0.00
产品分类：
病毒纯化试剂盒
公司分类：
Virus_purification_kit
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Assay Format
Number of Targets Detected:
100
Compatible Sample Types:
- Cell Culture Supernatants
- Cell Lysates
- Plasma
- Purified Proteins
- Serum
- Tissue Lysates
Design Principle:
- Label-based
- Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
- Semi-Quantitative
Solid Support:
Glass Slide
Product Specifications
Size:
4, 8, or 16 Sample Kit
Introduction
Glycocalyx, literally meaning ‘sugar coat’, is an extracellular polymeric coating surrounding many prokaryotic and eukaryotic cells consisting of glycoproteins, glycolipids, proteoglycans and glycosaminoglycans. The constituents of the glycocalyx play an important role in the process of cell signaling, virus infections, and immunity. However, detection tools for the research of glycobiology are currently in very limited supply.Raybiotech has pioneered the development of antibody arrays which are now widely applied in the research community with thousands of peer reviewed publications, including Cell and Nature. Taking advantage of advancements in microarray technology developed for antibody arrays, we have developed the first commercially available glycan array for screening protein-carbohydrate interactions. This array will help researchers:
- Identify the glycans binding partners in biological samples
- Identify whether target proteins are carbohydrate binding proteins
- Probe binding of viruses and whole cells to glycans1-3
- Profile the substrate specificity of enzymes (glycosyltransferases, glycosidases, etc.)4-6
- Profile the inflammatory immune response
The 100 synthetic glycans featured in the Glycan Array 100 are the most frequently identified structures showing important binding function in the literature. For example, influenza virus binds to a variety of sialosides with a serotype-specific pattern.7-9 Galectins, which are involved in apoptosis, cell adhesion and T-cell activation suppression, function by binding beta-galactosides.10-12
Glycan Names (100):Click Here for the Glycan Chart
| β-Glc- | β-Gal- | α-Man- | α-Fuc- |
| α-Rha- | β-GlcNAc- | β-GalNAc- | Tobramycin |
| Gal-β-1,3-GlcNAc-β- | Gal-α-1,3-Gal-β-1,3-GlcNAc-β- | Neu5Ac-α-2,3-Gal-β-1,3-GlcNAc-β- | Neu5Ac-α-2,6-Gal-β-1,3-GlcNAc-β- |
| Neu5Gc-α-2,3-Gal-β-1,3-GlcNAc-β- | Neu5Gc-α-2,6-Gal-β-1,3-GlcNAc-β- | Gal-β-1,3-(Fuc-α-1,4)-GlcNAc-β- [Lewis A] | Gal-β-1,4-Glc-β- |
| Gal-α-1,3-Gal-β-1,4-Glc-β- | Gal-α-1,4-Gal-β-1,4-Glc-β- | GlcNAc-β-1,3-Gal-β-1,4-Glc-β- | GalNAc-β-1,3-Gal-β-1,4-Glc-β- |
| Neu5Ac-α-2,3-Gal-β-1,4-Glc-β- | Neu5Ac-α-2,6-Gal-β-1,4-Glc-β- | Neu5Gc-α-2,3-Gal-β-1,4-Glc-β- | Neu5Ac-α-2,6-Gal-β-1,4-Glc-β- |
| Gal-β-1,4-(Fuc-α-1,3)-Glc-β- | GalNAc-β-1,3-Gal-α-1,4-Gal-β-1,4-Glc-β- | GlcNAc-β-1,6-GlcNAc-β- | 4-P-GlcNAc-β-1,4-Man-β-. |
| Glc-α-1,2-Gal-α-1,3-Glc-α- | Gal-β-1,3-GalNAc-α- | Gal-β-1,4-GlcNAc-β- | Gal-β-1,4 -(Fuc-α-1,3)-GlcNAc-β- [Lewis X] |
| Neu5Ac-α-2,3-Gal-β-1,4-(Fuc-α-1,3)-GlcNAc-β- [Sialyl Lewis X] | Neu5Ac-α-2,3-Gal-β-1,3 -(Fuc-α-1,4)-GlcNAc-β- [Sialyl Lewis A] | Neu5Gc-α-2,3-Gal-β-1,3-(Fuc-α-1,4)-GlcNAc-β- [Sialyl Lewis A] | Gal-α-1,4-Gal-β-1,3-GlcNAc-β- |
| Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-β- [LNnT] | GlcA-β-1,4-GlcNAc-α-1,4-GlcA-β- | GlcNAc-β-1,6-(Gal-β-1,3)-GalNAc-α-O-Ser | Neu5Ac-α-2,3Gal-β-1,4-(6S)GlcNAc-β- |
| GalNAc-β-1,4-GlcNAc-β- | Neu5Ac-α-2,8-Neu5Ac-α-2,3-Gal β-1,4-Glc-β- | Neu5Gc-α-2,8-Neu5Ac-α-2,3-Gal-β-1,4-Glc-β- | GalNAc-α-1,3-(Fuc-α-1,2)-Gal-β-1,4-Glc-β- [Blood A antigen tetrose] |
| GlcNAc-β-1,2-Man-α- | Neu5Ac-α-2,3-Gal-β- | Gal-β-1,3 -GalNAc-β-1,3-Gal-β- | Glc-α-1,2-Gal-α- |
| Gal-β-1,4-(Fuc-α-1,3)-GlcNAc-β-1,3-Gal-β- | Neu5Ac-α-2,3-Gal-β-1,4-(Fuc-α-1,3)-Glc-β- [3-Sialyl-3-fucosyllactose/ F-SL] | GlcNAc-β-1,4-GlcNAc-β- | β-D-GlcA- |
| Gal-β-1,4-(6S)GlNAc-β- | GlcNAc-α-1,3-(Glc-α-1,2-Glc-α-1,2)-Gal-α-1,3-Glc-α- | Gal-β-1,3-GalNAc-β-1,4-(Neu5Gc-α-2,3)-Gal-β-1,4-Glc-β- | Sisomicin Sulfate |
| GalNAc-α-1,3-(Fuc-α-1,2)-Gal-β- [Blood A antigen trisaccharide] | Fuc-α-1,2-Gal-β-1,4-GlcNAc-β- [Blood H antigen trisaccharide] | Gal-α-1,3-(Fuc-α-1,2)-Gal-β- [Blood B antigen trisaccharide] | Fuc-α-1,2-Gal-β-1,3-GlcNAc-β-1,3-Gal-β-1,4-Glc-β- [LNFP I] |
| Fuc-α-1,2-Gal-β-1,4-Glc-β- [Blood H antigen trisaccharide] | Gal-α-1,3-(Fuc-α-1,2)-Gal-β-1,4-Glc-β- [Blood B antigen tetrasaccharide] | (Fuc-α-1,2)-Gal-β-1,4-(Fuc-α-1,3)-GlcNAc-β- [ Lewis Y] | (Fuc-α-1,2)-Gal-β-1,3-(Fuc-α-1,4)-GlcNAc-β- [ Lewis B] |
| Gal-β-1,3-(Fuc-α-1,4)-GlcNAc-β-1,3-Gal-β-1,4-(Fuc-α-1,4)-Glc-β- [Lewis A] | Gal-β-1,3-GalNAc-β- | Gal-β-1,3-(Neu5Ac-α-2,6)-GalNAc-β- | Neu5Ac-α-2,6-Gal-β-1,3-GalNAc-β- |
| Neu5Ac-α-2,6-Gal-β-1,3-(Neu5Ac-α-2,6)-GalNAc-β- | Neu5Ac-α-2,3-Gal-β-1,3-(Neu5Ac-α-2,6)-GalNAc-β- | Neu5Ac-α-2,6-(Neu5Ac-α-2,3)-Gal-β-1,3-GalNAc-β- | GalNAc-β-1,4-(Neu5Ac-α-2,3)-Gal-β-1,4-Glc-β- [GM2] |
| GalNAc-β-1,4-(Neu5Ac-α-2,8-Neu5Ac-α-2,3)-Gal-β-1,4-Glc-β- [GD2] | Gal-α-1,4-Gal-β-1,4-GlcNAc-β- | β-D-Rha- | Glc-α-1,4-Glc-β- |
| Glc-α-1,6-Glc-α-1,4-Glc-β- | Maltotriose-β- | Glc-α-1,6-Glc-α-1,6-Glc-β- | Maltotetraose-β- |
| GlcNAc-α-1,4-GlcA-β-1,4-GlcNAc-α1,4-GlcA-β- | Maltohexaose-β- | Maltoheptaose-β- | Acarbose-β- |
| D-pentamannuronic acid-β- | L-pentaguluronic acid-β- | D-cellose-β- | Gal-α-1,3-Gal-β- |
| β-1,4-Xylotetrose | Chitin-trisaccharide | KDN-α-2,8-Neu5Ac-α-2,3-Gal-β-1,4-Glc-β- | Neu5Ac-α-2,8-Neu5Gc-α-2,3-Gal-β-1,4-Glc-β- |
| Neu5Ac-α-2,8-Neu5Ac-α-2,8-Neu5Ac-α-2,3-Gal-β-1,4-Glc-β- | Neu5Ac-α-2,8-Neu5Ac-α-2,6-Gal-β-1,4-Glc- | Gal-β-1,3-GalNAc-β-1,4-(Neu5Ac-α-2,3)-Gal-β-1,4-Glc-β- | Gentamicin Sulfate |
| Kanamycin sulfate | Geneticin Disulfate Salt (G418) | Neomycin trisulfate | SGP |
Application Data
Kit Components
- Dialysis Vials
- Labeling Reagent
- Stop Solution
- Glycan Array Glass Slide Assembly
- Sample Diluent
- 20X Wash Buffer I
- 20X Wash Buffer II
- Cy3 equivalent dye-conjugated Streptavidin
- Slide Washer/Dryer
- Adhesive device sealer
- Labeling Buffer
- Floating Dialysis Rack
Other Materials Required
- Detection antibodies of interest (For sandwich-based method only)
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Distilled or de-ionized water
- 1.5ml Polypropylene microcentrifuge tubes
- Gene microarray scanner or similar laser fluorescence scanner
- Small plastic or glass containers
- KCl, NaCl, KH2PO4 and Na2HPO4 (For label-based method only)
- Beaker, stir plate and stir bar
Protocol Outline
- Dry the glass slide
- Block array surfaces
- Incubate samples (samples need to be biotinylated for the label-based approach)
- For the the sandwich-based principle, incubate with a detection antibody cocktailFor the label-based principle, incubate the labeled-streptavidin.
- Incubate with Cy3 Equivalent Dye-Streptavidin
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
Upon receipt, all components of the RayBiotech Glycan Array 100 kit should be stored at -20°C. After initial use, remaining reagents should be stored at 4°C to avoid repeated freeze-thaw cycles and may be stored for up to 3 months (Labeling Reagent, Item B, should be prepared fresh each time before use). Unused glass slides should be kept at -20°C and repeated freeze-thaw cycles should be avoided (slides may be stored for up to 6 months). The entire kit should be used within 6 months of purchase.
References
- Amonsen, M.; Smith, D. F.; Cummings, R. D.; Air, G. M. J Virol 2007, 81, 8341-8345.
- Paulson, J. C.; de Vries, R. P. Virus Res 2013, 178, 99-113.
- Stencel-Baerenwald, J. E.; Reiss, K.; Reiter, D. M.; Stehle, T.; Dermody, T. S. Nat Rev Microbiol 2014, 12, 739-749.
- Park, S.; Shin, I. Org Lett 2007, 9, 1675-1678.
- Pourceau, G.; Chevolot, Y.; Goudot, A.; Giroux, F.; Meyer, A.; Moules, V.; Lina, B.; Cecioni, S.; Vidal, S.; Yu, H.; Chen, X.; Ferraris, O.; Praly, J. P.; Souteyrand, E.; Vasseur, J. J.; Morvan, F. Chembiochem 2011, 12, 2071-2080.
- Laurent, N.; Voglmeir, J.; Flitsch, S. L. Chem Commun 2008, 4400-4412.
- Xu, R.; de Vries, R. P.; Zhu, X.; Nycholat, C. M.; McBride, R.; Yu, W.; Paulson, J. C.; Wilson, I. A. Science 2013, 342, 1230-1235.
- Xu, R.; McBride, R.; Nycholat, C. M.; Paulson, J. C.; Wilson, I. A. J Virol 2012, 86, 982-990.
- Stevens, J.; Chen, L. M.; Carney, P. J.; Garten, R.; Foust, A.; Le, J.; Pokorny, B. A.; Manojkumar, R.; Silverman, J.; Devis, R.; Rhea, K.; Xu, X.; Bucher, D. J.; Paulson, J. C.; Cox, N. J.; Klimov, A.; Donis, R. O. J Virol 2010, 84, 8287-8299.
- Stowell, S. R.; Arthur, C. M.; Mehta, P.; Slanina, K. A.; Blixt, O.; Leffler, H.; Smith, D. F.; Cummings, R. D. J Biol Chem 2008, 283, 10109-10123.
- Stowell, S. R.; Arthur, C. M.; Slanina, K. A.; Horton, J. R.; Smith, D. F.; Cummings, R. D. J Biol Chem 2008, 283, 20547-20559.
- Carlsson, S.; Oberg, C. T.; Carlsson, M. C.; Sundin, A.; Nilsson, U. J.; Smith, D.; Cummings, R. D.; Almkvist, J.; Karlsson, A.; Leffler, H. Glycobiology 2007, 17, 663-676.
- Montgomery M., Hull E. Alterations in the glycome after HDAC inhibition impact oncogenic potential in epigenetically plastic SW13 cells. BMC Cancer. 2019; 19: 79. doi: 10.1186/s12885-018-5129-4Species: HumanSample type: Cell Lysate
- Hwang H., Han J., Kim G., Han J. Functional Expression and Characterization of the Recombinant N-Acetyl-Glucosamine/N-Acetyl-Galactosamine-Specific Marine Algal Lectin BPL3. Mar Drugs. 2018 Jan 5;16(1). pii: E13. doi: 10.3390/md16010013.Species: N/ASample type: Purified Proteins
- Dan X., Liu W., Ng T. Development and Applications of Lectins as Biological Tools in Biomedical Research. Med Res Rev. 2016 Mar;36(2):221-47. doi: 10.1002/med.21363.Species: N/ASample type: N/A
- McCombs J., Diaz J., Luebke K., Kohler J. Glycan specificity of neuraminidases determined in microarray format. Carbohydr Res. 2016 Jun 16;428:31-40. doi: 10.1016/j.carres.2016.04.003. Species: N/ASample type: Neuraminidase Proteins
University of North Carolina10/03/2019, 10:30 AM
Manual
SDS
Analysis Tool
GAL
品牌介绍
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