RayBiotech/小鼠凋亡信号通路阵列C1/2样本试剂盒/AAM-APOSIG-1-2
商品编号：
AAM-APOSIG-1-2
品牌：
RayBiotech
市场价：
¥0.00
美元价：
0.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Antigen Information
Gene Symbols:
AKT1 | ATM | BAD | CASP3 | CASP7 | CDKN1B | CHEK1 | EIF2S1 | HSPB1 | MAP3K7| MAPK14 | MAPK8 | NFKBIA | RELA | SMAD2 | TP53 View more Hide
Assay Format
Pathway:
- Akt Signaling
- HER/ErbB Signaling
- HIF-1 alpha Signaling
- IGF Signaling
- JAK/STAT Signaling
- MAPK Signaling
- mTOR Signaling
- NFkB Signaling
- p53 Signaling
- PI3K-AKT Signaling
- PKC Signaling
- TGF-beta Signaling
Number of Targets Detected:
17
Species Detected:
- Mouse
Compatible Sample Types:
- Cell Culture Supernatants
- Cell Lysates
- Plasma
- Serum
- Tissue Lysates
Design Principle:
- Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
- Semi-Quantitative
Solid Support:
Membrane
Product Specifications
Size:
2, 4, or 8 Sample Kit
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
AKT (P-Ser473)
| ATM (P-Ser1981)
| BAD (P-Ser112)
| Caspase-3 (P-Asp175)
| Caspase-7 (P-Asp198)
|
CHK1 (P-Ser296)
| eIF-2a (P-Ser52)
| ERK1/2 (P-Thr202)
| HSP27 (P-Ser82)
| IKBa (P-Ser32)
|
JNK (P-Thr183)
| NF-kB P65 (P-Ser536)
| p27 (P-Thr198)
| p38 (P-Thr180/Tyr182)
| P53 (P-Ser15)
|
SMAD2 (P-Ser245)
| TAK1 (P-Ser412)
| |||
Typical Results
Figure 1: NIH/3T3 cells were grown to 80% confluency and then serum starved overnight. Cells were treated (left panel) with Camptothecin (CPT) for 16 hours. Data shown are from a 20 second exposure using a chemiluminescence imaging system.
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Mouse Apoptosis Signaling Pathway Antibody Array C1 Membranes
- Blocking Buffer
- Detection Antibody Cocktail
- 1,000X HRP-Anti-Rabbit IgG Concentrate
- 20X Wash Buffer I Concentrate
- 20X Wash Buffer II Concentrate
- 2X Cell Lysis Buffer Concentrate
- Detection Buffer C
- Detection Buffer D
- 8-Well Incubation Tray w/ Lid
- Protease Inhibitor Cocktail
- 100x Phosphatase Inhibitor Cocktail I
- Phosphatase Inhibitor Cocktail II
- Plastic Sheets
- Array Map Template
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP"s ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE"s ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Detection Antibody Cocktail
- Incubate with HRP-Conjugated anti-IgG
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
- Radulovic, Katarina, et al. "NLRP6 Deficiency in CD4 T Cells Decreases T Cell Survival Associated with Increased Cell Death."?The Journal of Immunology?203.2 (2019): 544-556.Species: MouseSample type: Cell Lysates
- Liu F, Qiu H, Xue M, et al. MSC-secreted TGF-? regulates lipopolysaccharide-stimulated macrophage M2-like polarization via the Akt/FoxO1 pathway.?Stem Cell Res Ther. 2019;10(1):345. Published 2019 Nov 26. doi:10.1186/s13287-019-1447-ySpecies: MouseSample type: Lysates
Yale University06/28/2019, 05:19 PM
Manual
SDS
Analysis Tool
品牌介绍
raybiotech致力于开发、研制、生产及销售以抗体芯片、ELISA/EIA、抗原抗体为主的酶免产品。率先开发了高品质的抗体和蛋白质芯片技术平台，蛋白质芯片能够同时有效地分析生物样品中参与炎症、血管生长、细胞生长、细胞凋亡及信号转导等数百种蛋白的表达水平。近400页的产品目录，主要涉及蛋白质芯片、抗体芯片、抗体、免疫试剂、诊断试剂等近万种，拥有先进的技术优势。许多发表在Nature、Nature Medicine、 Cell、 PNAS、Lancet等世界顶级期刊上的文章中均使用了瑞博奥的产品，公司的芯片技术和产品质量都得到了广泛的肯定。
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