RayBiotech/人类磷酸化途径分析阵列C55/2样本试剂盒/AAH-PPP-1-2
商品编号：
AAH-PPP-1-2
品牌：
RayBiotech
市场价：
¥0.00
美元价：
0.00
产品分类：
RACE试剂盒
公司分类：
RACE_Reagent_kit
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Antigen Information
Gene Symbols:
AKT1 | AKT1S1 | AMPK1 | AND | ATF2 | ATM | BAD | CDKN1B | CREB1 | EGFR| EIF2S1 | EIF4EBP1 | FOS | GSK3A | GSK3B | HDAC2 | HDAC4 | HSPB1 | JAK1 | JAK2 | JUN | MAP2K1 | MAP2K3 | MAP2K6 | MAP3K7 | MAPK14 | MAPK8 | MTOR | NFKBIA | PDPK1 | PTEN | PTPN11 | PTPN6 | RAF1 | RELA | RPS6 | RPS6KA1 | RPS6KA3 | RPS6KA4 | RPS6KA5 | RPS6KB1 | SMAD1 | SMAD2 | SMAD4 | SMAD5 | SRC | STAT1 | STAT2 | STAT3 | STAT5A | STAT6 | TBK1 | TP53 | TYK2 | ZAP70 View more Hide
Assay Format
Pathway:
- Akt Signaling
- JAK/STAT Signaling
- MAPK Signaling
- NFkB Signaling
- TGF-beta Signaling
Number of Targets Detected:
55
Species Detected:
- Human
Compatible Sample Types:
- Cell Culture Supernatants
- Cell Lysates
- Plasma
- Serum
- Tissue Lysates
Design Principle:
- Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
- Semi-Quantitative
Solid Support:
Membrane
Product Specifications
Size:
2, 4, or 8 Sample Kit
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
4E-BP1 (P-Thr36)
| AKT (P-Ser473)
| AMPKa (P-Thr172)
| ATF2 (P-Thr69/71)
| ATM (P-Ser1981)
|
BAD (P-Ser112)
| C-Fos (P-Thr232)
| c-Jun (P-Ser73)
| CREB (P-Ser133)
| EGFR (P-Ser1070)
|
eIF-2a (P-Ser51)
| ERK1/2 (P-Thr202/P-Tyr204/P-Tyr185/P-Tyr187)
| GSK-3 alpha (P-Ser21)
| GSK-3 beta (P-Ser9)
| HDAC2 (P-Ser394)
|
HDAC4 (P-Ser632)
| HSP27 (P-Ser82)
| IKBa (P-Ser32)
| JAK1 (P-Tyr1022)
| JAK2 (P-Tyr1007/1008)
|
JNK (P-Thr183)
| MEK (P-Ser217/221)
| MKK3 (P-Ser189)
| MKK6 (P-Ser207)(MEK6)
| MSK1 (P-Ser376)
|
MSK2 (P-Ser360)
| mTOR (P-Ser2448)
| NF-kB (P-Ser536)
| p27 (P-Thr198)
| p38 (P-Thr180/Tyr182)
|
P53 (P-Ser15)
| P70S6K (P-Thr421/Ser424)
| PDK1 (P-Ser241)
| PRAS40 (P-Thr246)
| PTEN (P-Ser380)
|
Raf-1 (P-Ser301)
| RPS6 (P-Ser235/236)
| RSK1 (P-Ser380)
| RSK2 (P-Ser386)
| SHP-1 (P-Ser591)
|
SHP2 (P-Tyr542)
| SMAD1 (P-Ser463/465)
| SMAD2 (P-Ser245/250/255)
| SMAD4 (P-Thr277)
| SMAD5 (P-Ser463/465)
|
Src (P-Tyr419)
| STAT1 (P-Ser727)
| STAT2 (P-Tyr689)
| STAT3 (P-Tyr705)
| STAT5 (P-Tyr694)
|
STAT6 (P-Tyr641)
| TAK1 (P-Ser412)
| TBK1 (P-Ser172)
| TYK2 (P-Tyr1054)
| ZAP70 (P-Tyr292)
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Phosphorylation Pathway Profiling Array C55 Membranes
- Blocking Buffer
- Detection Antibody Cocktail
- 500X HRP-Anti-Rabbit IgG Concentrate
- 20X Wash Buffer I Concentrate
- 20X Wash Buffer II Concentrate
- 2X Cell Lysis Buffer Concentrate
- Detection Buffer C
- Detection Buffer D
- 8-Well Incubation Tray w/ Lid
- Protease Inhibitor Cocktail
- 100x Phosphatase Inhibitor Cocktail I
- Phosphatase Inhibitor Cocktail II
- Plastic Sheets
- Array Map Template
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP"s ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE"s ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Detection Antibody Cocktail
- Incubate with HRP-Conjugated anti-IgG
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
- Zhang, Dan, et al. "MALAT1 is involved in the pathophysiological process of PCOS by modulating TGF? signaling in granulosa cells."?Molecular and cellular endocrinology?499 (2020): 110589.Species: HumanSample type: Cell Lysates
Kalya Pharma07/15/2019, 11:43 AM
Manual
SDS
品牌介绍
raybiotech致力于开发、研制、生产及销售以抗体芯片、ELISA/EIA、抗原抗体为主的酶免产品。率先开发了高品质的抗体和蛋白质芯片技术平台，蛋白质芯片能够同时有效地分析生物样品中参与炎症、血管生长、细胞生长、细胞凋亡及信号转导等数百种蛋白的表达水平。近400页的产品目录，主要涉及蛋白质芯片、抗体芯片、抗体、免疫试剂、诊断试剂等近万种，拥有先进的技术优势。许多发表在Nature、Nature Medicine、 Cell、 PNAS、Lancet等世界顶级期刊上的文章中均使用了瑞博奥的产品，公司的芯片技术和产品质量都得到了广泛的肯定。
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