RayBiotech/人类凋亡信号阵列C1/2样本试剂盒/AAH-APOSIG-1-2-免疫在线蚂蚁淘旗下平台

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商品详细RayBiotech/人类凋亡信号阵列C1/2样本试剂盒/AAH-APOSIG-1-2

RayBiotech/人类凋亡信号阵列C1/2样本试剂盒/AAH-APOSIG-1-2

商品编号： AAH-APOSIG-1-2

品牌： RayBiotech

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美元价： 0.00

产地：美国(厂家直采)

公司：

产品分类：靶向测序试剂盒

公司分类： Targeted_sequencing_kit

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Antigen Information

Gene Symbols:

Assay Format

Pathway:

Akt Signaling
HIF-1 alpha Signaling
IGF Signaling
JAK/STAT Signaling
MAPK Signaling
mTOR Signaling
NFkB Signaling
p53 Signaling
PI3K-AKT Signaling
TGF-beta Signaling
Wnt/beta-Catenin Signaling

Number of Targets Detected:

Species Detected:

Human

Compatible Sample Types:

Cell Culture Supernatants
Cell Lysates
Plasma
Serum
Tissue Lysates

Design Principle:

Sandwich-based

Method of Detection:

Chemiluminescence

Quantitative/Semi-Quantitative:

Semi-Quantitative

Solid Support:

Membrane

Product Specifications

Size:

2, 4, or 8 Sample Kit

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Akt (P-Ser473)(PKB (P-Ser473)) Gene Symbol: AKT1 PKB RAC Gene ID: 207 Accession #: P31749 ELISA Kit: RayBio Human/Mouse/Rat Phospho-Akt (S473) ELISA	ATM (P-Ser1981) Gene Symbol: ATM Gene ID: 472 Accession #: Q13315 ELISA Kit: RayBio Human Phospho-ATM (S1981) ELISA	BAD (P-Ser112) Gene Symbol: BAD BBC6 BCL2L8 Gene ID: 572 Accession #: Q92934 ELISA Kit: RayBio Human Phospho-BAD (S112) ELISA	Caspase-3 (Cleaved Asp175) Gene Symbol: CASP3 CPP32 Gene ID: 836 Accession #: P42574 ELISA Kit: RayBio Human/Mouse Cleaved-Caspase-3 (D175) ELISA	Caspase-7 (Cleaved Asp198) Gene Symbol: CASP7 MCH3 Gene ID: 840 Accession #: P55210
CHK1 (P-Ser296) Gene Symbol: CHEK1 CHK1 Gene ID: 1111 Accession #: O14757	CHK2 (P-Thr68) Gene Symbol: CHEK2 CDS1 CHK2 RAD53 Gene ID: 11200 Accession #: O96017 ELISA Kit: RayBio Human Phospho-Chk2 (T68) ELISA	eIF-2a (P-Ser52) Gene Symbol: EIF2A CDA02 MSTP004 MSTP089 Gene ID: 83939 Accession #: Q9BY44 ELISA Kit: RayBio Human Phospho-EIF2A (S52) ELISA	ERK1 (P-T202/Y204)/ERK2 (P-Y185/Y187) Gene Symbol: MAPK3 ERK1 PRKM3/MAPK1 ERK2 PRKM1 PRKM2 Gene ID: 5595/5594 Accession #: P27361/P28482 ELISA Kit: RayBio Human/Mouse/Rat Phospho-Erk1 (T202/Y204) + Phospho-Erk2 (Y185/Y187) and Total Erk1/2 ELISA	HSP27 (P-Ser82) Gene Symbol: HSPB1 HSP27 HSP28 Gene ID: 3315 Accession #: P04792 ELISA Kit: RayBio Human Phospho-HSP27 (S82) ELISA
IKBa (P-Ser32) Gene Symbol: NFKBIA IKBA MAD3 NFKBI Gene ID: 4792 Accession #: P25963 ELISA Kit: RayBio Human Phospho-I-kappa B alpha (S32) ELISA	JNK (P-Thr183/Tyr185) Gene Symbol: MAPK8 JNK1 PRKM8 SAPK1 SAPK1C Gene ID: 5599 Accession #: P45983 ELISA Kit: RayBio Human/Mouse/Rat Phospho-JNK (T183/Y185) ELISA	NFKBP65 (P-Ser536) Gene Symbol: RELA NFKB3 Gene ID: 5970 Accession #: Q04206 ELISA Kit: RayBio Human Phospho-NF-KB p65 (S536) ELISA	PARP1 (Cleaved Asp214/Gly215) Gene Symbol: PARP1 ADPRT PPOL Gene ID: 142 Accession #: P09874 ELISA Kit: RayBio Human Cleaved-PARP (D214/G215) ELISA	p27 (P-Thr198) Gene Symbol: PSMD9 Gene ID: 5715 Accession #: O00233 ELISA Kit: RayBio Human Phospho-P27/Kip1 (T198) ELISA
P53 (P-Ser15) Gene Symbol: TP53 P53 Gene ID: 6198 Accession #: P23443 ELISA Kit: RayBio Human/Mouse/Rat Phospho-P53 (S15) ELISA	SMAD2 (Ser245/250/255) Gene Symbol: SMAD2 MADH2 MADR2 Gene ID: 4087 Accession #: Q15796 ELISA Kit: RayBio Human/Mouse/Rat Phospho-SMAD2 (SER245/250/255) ELISA	TAK1 (P-Ser412) Gene Symbol: MAP3K7 TAK1 Gene ID: 6885 Accession #: O43318 ELISA Kit: RayBio Human Phospho-TAK1 (Ser412) ELISA

Typical Results

Figure 1: HeLa cells were grown to 80% confluency and then serum starved overnight. Cells were either untreated (bottom panel) or treated (top panel) with Staurosporine (STS) for 3 hours. Data shown are from a 20 second exposure using a chemiluminescence imaging system.

Figure 2: Jurkat cells were either untreated (bottom panel) or treated (top panel) with Camptothecin(CPT) for 16 hours. Data shown are from a 20 second exposure using a chemiluminescence imaging system.

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Human Apoptosis Signaling Antibody Array C1 Membranes
Blocking Buffer
Detection Antibody Cocktail
1,000X HRP-Anti-Rabbit IgG Concentrate
20X Wash Buffer I Concentrate
20X Wash Buffer II Concentrate
2X Cell Lysis Buffer Concentrate
Detection Buffer C
Detection Buffer D
8-Well Incubation Tray w/ Lid
Protease Inhibitor Cocktail
100x Phosphatase Inhibitor Cocktail I
Phosphatase Inhibitor Cocktail II
Plastic Sheets
Array Map Template
Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP"s ChemiDoc-It^® or EpiChem II Benchtop Darkroom or GE"s ImageQuant^™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Detection Antibody Cocktail
Incubate with HRP-Conjugated anti-IgG
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20^°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20^°C and all other reagents undiluted at 4^°C for no more than 3 months.

Bao X, Shi J, Xie F, et al. Proteolytic Release of the p75NTR?Intracellular Domain by ADAM10 Promotes Metastasis and Resistance to Anoikis.?Cancer Res. 2018;78(9):2262-2276. doi:10.1158/0008-5472.CAN-17-2789Species: Human Sample type: Cell Lysates
Smolensky D, Rhodes D, McVey DS, et al. High-Polyphenol Sorghum Bran Extract Inhibits Cancer Cell Growth Through ROS Induction, Cell Cycle Arrest, and Apoptosis.?J Med Food. 2018;21(10):990-998. doi:10.1089/jmf.2018.0008Species: HumanSample type: Cell Lysates

The human apoptosis array provides a fast and comprehensive glance about the key signaling involved in the apoptotic pathway. It's a one shot experiment with certain controls already on the blot, pretty simple to execute and normalize. Would recommend it.

VinayakMoffitt Cancer Center12/05/2019, 10:28 AM

This apoptosis kit was very user-friendly and gave detailed instructions on how to analyze findings using a software like Excel. This assay was very helpful for examining multiple gene targets simultaneously, so we got a lot of data out of 1 experiment. Negative and positive controls were included on the blots, which was useful in subsequent analyses.Notes: the Caspase3 antibody seemed a lot more sensitive than other antibodies on the blot. Also, some of the gene names in the kit manual differed from those listed on the website, so keep that in mind when analyzing data to ensure that the correct gene is being referred to.

Melanie GartzMedical College of Wisconsin10/09/2019, 12:51 PM

It seems antibodies are not evenly attached on the membrane. Some signals show up outside of spots. It has high background when I follow up the suggested procedures. I cannot see any benefit of this array compared to other array such as apoptosis array or other company's similar product.

SangPenn State College of Medicine02/15/2019, 01:54 PM

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Manual

SDS

Analysis Tool

品牌介绍

raybiotech致力于开发、研制、生产及销售以抗体芯片、ELISA/EIA、抗原抗体为主的酶免产品。率先开发了高品质的抗体和蛋白质芯片技术平台，蛋白质芯片能够同时有效地分析生物样品中参与炎症、血管生长、细胞生长、细胞凋亡及信号转导等数百种蛋白的表达水平。近400页的产品目录，主要涉及蛋白质芯片、抗体芯片、抗体、免疫试剂、诊断试剂等近万种，拥有先进的技术优势。许多发表在Nature、Nature Medicine、 Cell、 PNAS、Lancet等世界顶级期刊上的文章中均使用了瑞博奥的产品，公司的芯片技术和产品质量都得到了广泛的肯定。

公司介绍

品牌分类

\数组\ ELISA试剂盒其他产品

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