raybotech/人/小鼠MAPK磷酸化阵列/2样本试剂盒/AAH-MAPK-1-2
商品编号：
AAH-MAPK-1-2
品牌：
RayBiotech
市场价：
¥0.00
美元价：
0.00
产品分类：
多克隆抗体
公司分类：
Polyclonal_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Antigen Information
Gene Symbols:
AKT1 | CREB1 | GSK3A | GSK3B | HSPB1 | MAP2K1 | MAP2K3 | MAP2K6 | MAPK1 | MAPK14| MAPK8 | MTOR | RPS6KA1 | RPS6KA3 | RPS6KA4 | RPS6KB1 | TP53 View more Hide
Assay Format
Pathway:
- Akt Signaling
- HER/ErbB Signaling
- IGF Signaling
- JAK/STAT Signaling
- MAPK Signaling
- NFkB Signaling
- PI3K-AKT Signaling
- Wnt/beta-Catenin Signaling
Number of Targets Detected:
17
Species Detected:
- Human
- Mouse
Compatible Sample Types:
- Cell Culture Supernatants
- Cell Lysates
- Plasma
- Serum
- Tissue Lysates
Design Principle:
- Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
- Semi-Quantitative
Solid Support:
Membrane
Product Specifications
Size:
2, 4, or 8 Sample Kit
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
Akt (P-Ser473)(PKB (P-Ser473))
| CREB (P-Ser133)
| ERK1 (P-T202/Y204)/ERK2 (P-Y185/Y187)
| GSK3a (P-Ser21)
| GSK3b (P-Ser9)
|
HSP27 (P-Ser82)
| JNK (P-Thr183)
| MEK (P-Ser217/Ser221)
| MKK3 (P-Ser189)
| MKK6 (P-Ser207)
|
MSK2 (P-Ser360)
| mTOR (P-Ser2448)
| p38 (P-Thr180/Tyr182)
| P53 (P-Ser15)
| P70S6K (P-Thr421/Ser424)
|
RSK1 (P-Ser380)
| RSK2 (P-Ser386)
| |||
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human/Mouse MAPK Phosphorylation Antibody Array C1 Membranes
- Blocking Buffer
- Detection Antibody Cocktail
- 1,000X HRP-Anti-Rabbit-IgG Concentrate
- 20X Wash Buffer I Concentrate
- 20X Wash Buffer II Concentrate
- 2X Cell Lysis Buffer Concentrate
- Detection Buffer C
- Detection Buffer D
- 8-Well Incubation Tray w/ Lid
- Protease Inhibitor Cocktail
- 100x Phosphatase Inhibitor Cocktail I
- Phosphatase Inhibitor Cocktail II
- Plastic Sheets
- Array Map Template
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP??s ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE"s ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Detection Antibody Cocktail
- Incubate with HRP-Conjugated anti-IgG
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Typical Data
Figure 1 - HeLa cells were grown to 80% confluency and then serum starved overnight. Cells were either untreated (bottom panel) or treated (top panel) with 250 nM PMA for 20 minutes. Data shown are from a 20 second exposure using a chemiluminescence imaging system. 
Figure 2 - HepG2 cells were grown to 80% confluency and then serum starved overnight. Cells were either untreated (bottom panel) or treated (top panel) with 25 ng/mL of recombinant human IL-1β for 30 minutes. Data shown are from a 20 second exposure using a chemiluminescence imaging system. 
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
- Dhani, Shanel, et al. "Fusaric acid alters Akt and ampk signalling in c57bl/6 mice brain tissue."?Food and Chemical Toxicology?(2020): 111252.Species: MouseSample type: Tissue Lysates
- Kaiser, Franziska, et al. "Choice of factors and medium impinge on success of ESC to TSC conversion."?Placenta?90 (2020): 128-137.Species: HumanSample type: Cel Lysates
- Sarsour, Ehab H., et al. "Arachidonate 12-lipoxygenase and 12-hydroxyeicosatetraenoic acid contribute to stromal aging-induced progression of pancreatic cancer."?Journal of Biological Chemistry?295.20 (2020): 6946-6957.Species: Human Sample type: Cell Lusates
- L'Huillier C, Jarbeau M, Achamrah N, et al. Glutamine, but not Branched-Chain Amino Acids, Restores Intestinal Barrier Function during Activity-Based Anorexia.?Nutrients. 2019;11(6):1348. Published 2019 Jun 15. doi:10.3390/nu11061348Species: MouseSample type: Tissue Lysates
- Che G., Wang Y., Zhou B., et al. Knockdown of Heparanase Suppresses Invasion of Human Trophoblasts by Activating p38 MAPK Signaling Pathway. Dis Markers. 2018 Apr 17;2018:7413027. doi: 10.1155/2018/7413027. Species: HumanSample type: Cell Lysate
- Yu S., Zhong Q., Chen R., et al. CRLF1 promotes malignant phenotypes of papillary thyroid carcinoma by activating the MAPK/ERK and PI3K/AKT pathways. Cell Death Dis. 2018 Mar 7;9(3):371. doi: 10.1038/s41419-018-0352-0.Species: HumanSample type: Cell Lysate
- Hou J, Cui C, Kim S, Sung C, Choi C. Ginsenoside F1 suppresses astrocytic senescence-associated secretory phenotype.?Chem Biol Interact. 2018;283:75-83. doi:10.1016/j.cbi.2018.02.002Species: HumanSample type: Cell Lysates
- Che, Guanglu et al. ?Knockdown of Heparanase Suppresses Invasion of Human Trophoblasts by Activating p38 MAPK Signaling Pathway.??Disease markers?vol. 2018 7413027. 17 Apr. 2018, doi:10.1155/2018/7413027Species: HumanSample type: Cell Lysates
- Yu, Shi-Tong, et al. "CRLF1 promotes malignant phenotypes of papillary thyroid carcinoma by activating the MAPK/ERK and PI3K/AKT pathways."?Cell death & disease?9.3 (2018): 1-13.Species: HumanSample type: Cell Lysates
- Poling, Holly M., et al. "Mechanically induced development and maturation of human intestinal organoids in vivo."?Nature biomedical engineering?2.6 (2018): 429-442.Species: HumanSample type: Tissue Lysates
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