RayBiotech/人类炎症阵列Q1/8样本试剂盒/QAH-INF-1-1
商品编号：
QAH-INF-1-1
品牌：
RayBiotech
市场价：
¥0.00
美元价：
0.00
产品分类：
胶原酶
公司分类：
Collagenase
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Antigen Information
Gene Symbols:
- CCL2
- CXCL8
- IFNG
- IL10
- IL13
- IL1A
- IL1B
- IL4
- IL6
- TNF
Assay Format
Pathway:
- JAK/STAT Signaling
- MAPK Signaling
- NFkB Signaling
Number of Targets Detected:
10
Species Detected:
- Human
Compatible Sample Types:
- Cell Culture Supernatants
- Cell Lysates
- Other Body Fluids
- Plasma
- Serum
- Tissue Lysates
Design Principle:
- Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
- Quantitative
Solid Support:
Glass Slide
Product Specifications
Size:
1, 2, or 4 Slide Kit (16 sub-arrays per slide)
Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
IFN-gamma
| IL-1 alpha(IL-1 F1)
| IL-1 beta(IL-1 F2)
| IL-10
| IL-13
|
IL-4
| IL-6
| IL-8(CXCL8)
| MCP-1(CCL2)
| TNF alpha
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Human Inflammation Array Q1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
- Zhang C, Ding H, He M, Liu L, Liu L, Li G, et al. (2016) Comparison of Early Changes in Ocular Surface and Inflammatory Mediators between Femtosecond Lenticule Extraction and Small-Incision Lenticule Extraction. PLoS ONE 11(3): e0149503. doi:10.1371/journal.pone.0149503Species: HumanSample type: Tears
- Wu B., Wang W., Zhan Y., et al. CXCL13, CCL4, and sTNFR as circulating inflammatory cytokine markers in primary and SLE-related autoimmune hemolytic anemia. J Transl Med. 2015 Apr 8;13:112. doi: 10.1186/s12967-015-0474-4.Species: HumanSample type: Plasma
- Modulation of Cytokine Release and Gene Expression by the Immunosuppressive Domain of gp41 of HIV-1Species: HumanSample type: N/A
- Provençal, Nadine, et al. "Childhood chronic physical aggression associates with adult cytokine levels in plasma."?PLoS One?8.7 (2013): e69481.Species: HumanSample type: Plasma
- Provençal, Nadine, et al. "Differential DNA methylation regions in cytokine and transcription factor genomic loci associate with childhood physical aggression."?PLoS One?8.8 (2013): e71691.Species: HumanSample type: Plasma
- Zhai Y, Zhong Z, Chen C-YA, Xia Z, Song L, Blackburn MR, Shyu A-B. Coordinated Changes in mRNA Turnover, Translation, and RNA Processing Bodies in Bronchial Epithelial Cells following Inflammatory Stimulation. Mol Cell Biol. 2008; 28(24):7414-7426.Species: HumanSample type: N/A
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品牌介绍
raybiotech致力于开发、研制、生产及销售以抗体芯片、ELISA/EIA、抗原抗体为主的酶免产品。率先开发了高品质的抗体和蛋白质芯片技术平台，蛋白质芯片能够同时有效地分析生物样品中参与炎症、血管生长、细胞生长、细胞凋亡及信号转导等数百种蛋白的表达水平。近400页的产品目录，主要涉及蛋白质芯片、抗体芯片、抗体、免疫试剂、诊断试剂等近万种，拥有先进的技术优势。许多发表在Nature、Nature Medicine、 Cell、 PNAS、Lancet等世界顶级期刊上的文章中均使用了瑞博奥的产品，公司的芯片技术和产品质量都得到了广泛的肯定。
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